102 PROPERTIES OF ILEMOLYTIC SERA 



immune-body to the guinea-pig's immune-bodies, which was 

 obtained by injecting a rabbit with guinea-pig's serum. It 

 is convenient, as Bordet has done, to indicate that a serum 

 has been deprived of the action of complement by heating, 

 to place 55 C. after its name, e.g. guinea-pig's serum 55 

 means the serum of a normal guinea-pig, heated for an hour 

 at 55 C. 



DEMONSTRATIONS OF THE ACTION OF ANTI-IMMUNE-BODY 



As stated above, the anti-immune-body is obtained by 

 injecting the guinea-pig with the normal serum of the rabbit. 

 We shall speak of such a serum obtained from a guinea-pig 

 as anti-IB; it is, of course, deprived of complement by 

 heating at 55 C. 



The fundamental result obtained by Bordet, showing that 

 the anti-immune-body unites with red corpuscles sensitized 

 with immune-body and protects them against the action of 

 complement, is readily demonstrated. The following may 

 be taken as an example. 



The experiment may be represented thus : 



\W \W 



A. ECs + 3 IB j + 0-3 c.c. anti-IB ; + C no lysis I 



B. KCs + 3 IB | +0-3 c.c. guinea-pig's serum 55 j + C lysis l 



The vertical interrupted line represents a period of incubation at 

 37 C., for an hour unless the time is stated. 



W signifies that the corpuscles are washed and centrifugalized 

 after incubation. 



Two series of tubes (A and B) each containing 0-5 c.c. of 

 suspension of red corpuscles are prepared, and to each 

 three haemolytic doses of immune-body are added. After 

 an hour to allow for combination the corpuscles are centri- 

 fugalized and washed with salt solution (all the immune- 

 body present is therefore in combination with the red 

 1 The corpuscles are suspended in guinea-pig's serum 55 C. 



