ON ANTI-IMMUNE-BODIES 105 



time in series B it was complete in the tube containing -015 c.c. 

 of complement ; at the end of forty minutes, however, lysis 

 was complete in all the tubes of both series. The action of 

 anti-IB can thus be demonstrated quite clearly by the rate 

 of occurrence of lysis when the corpuscles are suspended in 

 salt solution, even though lysis is not prevented. The 

 difference as regards lysis in the two media is due to the 

 fact that a much larger dose of complement is necessary 

 when the corpuscles are suspended in guinea-pig's serum 55; 

 that is, complement acts more feebly, and any further 

 diminution in its action is much more apparent. We there- 

 fore conclude that whether lysis is prevented or not, the 

 anti-immune-body in every case keeps a certain amount of 

 complement out of combination. 



ACTION OF ANTI-IMMUNE-BODY ON MULTIPLE IMMUNE- 

 BODIES DEVELOPED BY ACTIVE IMMUNIZATION 



Bordet found that anti-immune-body acted on all the 

 immune-bodies supplied by the animal whose serum was 

 injected in order to produce the anti-immune-body. In 

 addition to the immune-body for ox's corpuscles we have 

 also investigated by quantitative methods the action of 

 anti-immune-body in the case of the immune-body for 

 guinea-pig's corpuscles (obtained by injecting a rabbit with 

 these corpuscles). 



Example. Dose of IB for guinea-pig's corpuscles = 

 0-006 c.c. 1 



Two series of tubes, A and B, containing 0-5 c.c. suspension 

 of red corpuscles and four doses of immune-body, are 

 prepared as before, those in A also receiving 0-2 c.c. anti-IB 

 the details need not be repeated. In series A the amount 

 of guinea-pig's complement taken up was 0-015 c.c. ; in 

 series B, 0-045 c.c. Therefore the anti-immune-body (0- 2 c.c. ) 



1 i.e. along with rabbit's complement. On variations in dosage vide 

 p. 72. 



