ON H^MOLYTIC RECEPTORS 117 



lysis, was used. His results, therefore, go to show that the receptors 

 remain attached to the stromata. He used the corpuscles of the 

 rabbit and the corresponding immune-body obtained from the 

 guinea-pig. The latter animal was also employed for testing the 

 result of the injection of stromata. Nolf 1 (1900), on the other hand, 

 arrived at a conclusion quite contrary to that of Bordet. He employed 

 the corpuscles of the fowl, and the injections were made into the 

 rabbit. He produced lysis with water, then made up with sodium 

 chloride, and separated the stromata from the fluid by centri- 

 fugalization. He found by this means that the injection of the 

 stromata gave rise to agglutinin but little lysin, whilst the cellular 

 contents (contenu gldbulaire), which were dissolved out into the 

 fluid, had the converse property, giving rise to much lysin but little 

 agglutinin. According to his results, most of the hsemolytic receptors 

 are separated from the stromata on lysis with water. G. N. Stewart 2 

 (1904) arrives at results which to a certain extent resemble those of 

 Nolf, inasmuch as they point to a closer relation between the agglu- 

 tinogenic property and the stromata than between the lysinogenic 

 property and the stromata ; he considers, however, that Nolf J s 

 decided conclusions are not justified. He finds that both the stro- 

 mata and the haemoglobin-containing fluid obtained after lysis have 

 the property of giving rise to agglutinins and lysins on injection, 

 but that the agglutinating property is more marked when the 

 stromata are injected, the hsemolytic property when the separated 

 fluid is used. When milder hsemolytic agents are used, there is less 

 separation of the agglutinogenic receptors from the stromata than 

 when water is used. In most of his experiments he used rabbits' 

 corpuscles, and the injections were made into guinea-pigs ; the 

 observations are thus comparable with those of Bordet, and the 

 variance in the results is noteworthy. 



From this resume it will be seen that the results obtained 

 by the different observers are of conflicting nature, and it 

 cannot be considered satisfactorily determined whether the 

 receptors of the red corpuscles pass into the surrounding 

 fluid or remain attached to the stromata when the corpuscles 

 are lysed, say with water. All the observers mentioned 



1 Nolf, Annales de Vlnst. Pasteur, 1900, vol. xiv, p. 297. 



8 G. N. Stewart, Amer. Journ. Physiol., Boston, 1904, vol. xi, p. 250. 



