122 PROPERTIES OF ILEMOLYTIC SERA 



EFFECTS OF HEAT ON THE RECEPTORS 



We have also carried out in a similar manner experiments 

 on the effects of exposing the corpuscles to various tempera- 

 tures, and may give briefly the results of these. The cor- 

 puscles were laked with water, and sodium chloride was 

 added to make up to 0-85 per cent. The red fluid was 

 exposed to various temperatures, the same amount of 

 corpuscles of course being used in each case. Examples : 



1. Six hsemolytic doses of immune-body added to each tube. 



Hsemolytic dose of complement = 0-015 c.c. 

 1 c.c. of unheated suspen- 

 sion . . . takes up 0- 12 c.c. of complement. 

 of suspension heated 



for ten minutes at 



65 C . . . 0-09 c.c. 

 of suspension heated 



for ten minutes at 



75 C . . . 0-07 c.c. 

 of suspension heated 



for ten minutes at 



85 C . . . 0-05 c.c. 



2. Six haemolytic doses of immune-body, &c., as in 1. 



1 c.c. of suspension heated 

 for sixty minutes at 



85 C. . . . takes up 0-042 c.c. of complement. 

 of suspension heated 

 for ten minutes at 

 100 C. ... 0-045 c.c. 



3. Six haemolytic doses of immune-body. Hseniolytic dose of 



complement = 0-02 c.c. 

 1 c.c. of unheated suspen- 

 sion . . . takes up 0-17 c.c. of complement. 

 of suspension heated 

 for ninety minutes 

 at 100 C. . . 0-045 c.c. 



Controls were made without any immune-body ; in these 

 there was no appreciable absorption of complement. 



