DEVIATION OF COMPLEMENT 151 



Anti-human serum 0-05 c.c. to each tube. 



TABLE 5 



Amount of Chimpanzee 



serum Human serum serum Macacus serum 



0-000,1 c.c. ? Slight opalescence 



0-001 Distinct precipitate Slight but distinct ? Slight opalescence 

 0-01 Marked Marked, less than Distinct though slight 



with human 



These results are much in conformity with what Nuttall l 

 obtained. 



Whilst, however, the deviation test places the chimpanzee 

 and the macacus monkey in practically the same relation 

 to man, the precipitation test brings out a difference, the 

 chimpanzee serum giving a more marked reaction with 

 anti-human serum than the macacus serum does. No doubt 

 other analogous results will be found to obtain. 



We have also tested the anti-ox serum with the sera of 

 some other animals. Using 0-01 c.c. and 0-001 c.c. of the 

 serum to be tested, we obtained no deviation with the 

 serum of the horse, pig, cat, mouse, pigeon and of man. 

 (Larger amounts of serum than those mentioned were not 

 used, as complications may arise from the added serum 

 interfering with lysis.) With the serum of the sheep, 

 however, a deviation was obtained approximating in degree 

 to that given by ox's serum. With 0-05 c.c. anti-ox serum, 

 0-000,01 c.c. sheep's serum as well as 0-000,01 c.c. ox's serum 

 gave a slight though distinct deviation of guinea-pig's 

 complement, but this was more marked in the case of the 

 ox's serum. A greater difference was, however, brought 

 out when we tested the amount of deviation with a larger 

 amount of the two sera, viz. 0-001 c.c. (the same quantity 

 of anti-serum, 0-05 c.c., being used). In this test we found 

 that six hsemolytic doses of guinea-pig's complement in the 

 case of the ox serum and four haemolytic doses in the case 

 of the sheep's serum, had to be added before one free dose 



1 Nuttall, Blood Immunity and Relationship, Cambridge, 1904, p. 165. 



