154 PROPERTIES OF ANTI-SERUM TO SERUM 



above as well as below that optimum the amount of devia- 

 tion diminishes. The maximum precipitate was given by 

 0-01 c.c. The results of this experiment are graphically 

 represented in Figure 2. 



Amount of guinea-pig's complement 

 q-' b 



Anti-serum -025 c.c. 

 + serum -1 c.c. 



-f serum -01 c.c. 



,, + serum -001 c.c. 



-f serum -000,1 c.c. 



-f serum -000,01 c.c. 



Fig. 2. This figure shows the amount of deviation of complement with 

 O025 c.c. of anti-serum and varying amounts of homologous serum. The 

 maximum deviation is given with 0-001 c.c. 



curve a = initial lysis, 

 b = half lysis, 

 c = complete lysis. 



A similar result was obtained with anti-human serum, 

 as is shown in the following table. 



Anti-serum rabbit v. man, -025 c.c. to each tube. Varying amounts of 

 human serum. 



TABLE 7 

 Human 

 Anti- serum Guinea-pig's complement 



serum 55 C. ' > 



c.c. c.c. 0-01 0-02 0-03 0-04 0-05 cc. 



0*025 0-1 complete complete complete \ 



0-025 0-01 complete complete Lysis in 



0-025 0-001 completed added 



0-025 0-000,1 complete complete complete corpuscles 



0-025 0-000,01 complete complete complete completej 



In this experiment there was some diffusion of haemo- 

 globin owing to the corpuscles having been kept too long ; 

 the tubes showing complete lysis are accordingly only given. 

 The maximum deviation was thus given by 0-001 c.c. of 



