200 ANTI-BACTERIAL PROPERTIES OF SERUM 



EXAMPLE 3. Inoculation with 0-0005 c.c. 24-hour 'bouillon 

 culture of 7. Metchnikovi. 



Result. 0:01 c.c. gives no reduction, 0-1 c.c. distinct reduction. 



EXAMPLE 4. -Inoculation with 0-00006 c.c. 24-hour bouillon 

 culture of B. Gaertneri. 



Result. 0-01 c.c. gives distinct reduction, 0-1 c.c. great reduction. 

 It is to be noted that the V. cholerce is a very active absorber of 

 bactericidal complement, a result which Buxton also obtained. 



The following table gives the general results of experi- 

 ments with heterologous organisms ; it shows (a) the dead 

 organism whose absorbing effect is to be tested, (b) the 

 smallest amount of culture which gives a distinct reduction 

 of bactericidal action, and (c) the living organism on which 

 the treated serum was tested : 



