138 



Prof. B. Moore. On the Synthesis of [June 15, 



The neutral alcoholic solution thus obtained was next evaporated 

 down to a small volume, a measured volume (usually 10 cc.) of standard 

 alcoholic potash solution was added, and the mixture boiled for 20 30 

 minutes, in a small flask fitted with a reflux tube, in order to insure 

 complete saponification of all the neutral fat present.* Finally 

 the contents of the flask were accurately neutralised by standard 

 hydrochloric acid, with phenol-phthalein as indicator. The difference 

 in value between alkali added before boiling and acid required after- 

 wards then supplied the necessary datum for calculating the percentage 

 of neutral fat. 



Expt. 1. A dog, weighing 12 kilos., was fed with 100 grammes of olive oil at 

 9.30 A.ic. and anaesthetised at 3.30 P.M., when the lacteals were exposed, and 

 seventeen drawn-out tubes were filled with milk-white chyle by opening the 

 lacteals. 



Grammes. 



Weight of lymph = 1-2990 



total ethereal extract. . = 0'0618 



free fatty acid = 0'0028 



neutral fat = 0'0564 



Percentage of free fatty acid .... = 4' 7 

 neutral fat = 95'3 



Expt. 2. Weight of dog = 8'6 kilos., fed with 50 grammes of olive oil at 

 9.30 A.M. ; chyle collected as before at 4.30 P.M., and twenty -three tubes charged 

 with the fluid. 



The lymph was weighed and analysed with the following results : 



Weight of lymph 



ethereal extract 



free fatty acid 



neutral fat 



Percentage of free fatty acid .... 



neutral fat . . 



Grammes. 

 0-9560 

 ' 0-1052 

 0-0042 

 0*1031 



3-9 

 = 96-1 



Expt. 3.-Weight of dog = 12-4 kilos., fed at 9 A.M. with 100 grammes of olive 

 oil ; chyle collected at 3 P.M. by a fine pipette into a porcelain capsule, and then 

 weighed and analysed. 



Grammes. 



Weight of lymph = 1-8712 



ethereal extract = 0'1450 



free fatty acid = 0*0042 



neutral fat = 0'1326 



Percentage of free fatty acid = 3'1 



neutral fat = 96'9 



Hence in these experiments practically all the fatty matter (upwards 

 of 95 per cent.) is present in neutral fat. 



* Kottstorfer's method, as described in Button's ' Volumetric Analysis,' 8th Ed., 

 p. 402. Preliminary experiments to test the method with pure neutral olein gave 

 practically theoretical values. 



