1903.] Fats accompanying Absorption from the Intestine. 141 



which formed a temporary emulsion on shaking, and the fluid, examined under a 

 low power of the microscope, showed a field thickly studded over with highly 

 refractile globules, closely resembling milk, as seen under the microscope. 



The oily layer was removed by shaking up with successive quantities of ether, in 

 which it was readily soluble, and after evaporation of the ether, the amount of 

 ethereal extractive was weighed, and the percentage of free oleic acid in it was 

 determined by titration with decinormal sodic hydrate, in warm alcoholic solution, 

 using rosolic acid as indicator. It was found that 87' 5 per cent, of the ethereal 

 extract consisted of free oleic acid. 



The lymphatic extract used was alkaline to rosolic acid and remained so during 

 the reaction. 



Expt. 3. This experiment shows that the production of free acid from soap is 

 not stopped by the prolonged action of sulphuretted hydrogen upon the tissue cells 

 or extracts. 



Fresh ox lymphatic glands were treated as above described, and then extracted 

 in a flask, with 5 volumes of distilled water, which was saturated with sulphuretted 

 hydrogen gas, and then tightly corked and kept in an incubator at 36 C. for 

 92 hours. 



At the end of the interval the contents still had a strong odour of sulphuretted 

 hydrogen. The fluid was separated from the tissue elements, and a water clear 

 extract of a greenish-brown colour was obtained. 



The fluid was charged again with sulphuretted hydrogen and left at room tem- 

 perature, tightly corked for another 10 days. It was then filtered from a slight 

 deposit of sulphur, and found to be slightly acid (acidity = 0'24 N). It was 

 rendered alkaline (alkalinity = 0*02 N to rosolic acid) by excess of sodium car- 

 bonate. 



A portion of 40 c.c. had 0'8 gramme sodium oleate and 0'4 gramme of glycerine 

 added ; the flask was saturated with sulphuretted hydrogen, corked and placed in 

 the incubator at 36 C. for 24 hours when an oily layer had appeared on the surface. 

 A single extraction with ether gave a residue weighing 0'355 gramme and contain- 

 ing 0'341 gramme of free oleic acid. 



Expt. 4. Cell-free extracts of the pancreas and small intestine (mucosa) of 

 three cats, taken in condition of inanition, prepared as before, and digested with 

 normal saline for a period of 43 hours in an incubator at 36 C. The solutions 

 were made distinctly alkaline to phenol-phthalein, and the strengths were made 

 equal to 1 in 9 of the fresh tissues. 



Each extract (intestine and pancreas) was then measured out into four portions, 

 each of 25 c.c., and the two sets of solutions were treated as follows : 



No. 1. 25 c.c. extract + 0'5 gramme oleate + 0'1 gramme dextrose + 0'16 gramme 



glycerine. 



No. 2. 25 c.c. extract -t-0'5 gramme oleate + 0*16 gramme glycerine. 

 No. 3. 25 c.c. extract + 0*5 gramme oleate. 

 No. 4. 25 c.c. extract + 0'5 gramme oleate + boiling before digestion. 



The eight tubes were then placed in a water-bath at 36 C. and examined at 

 intervals. 



Intestine, 



An examination Ifc hours after the commencement of the experiment showed 

 turbidity in all four of the intestinal extract tubes, and under the microscope 

 thickly covered fields of oil globules. 



The experiment was concluded at the end of 19 hours 35 minutes for analyses of 

 the contents as given below, and at this time each tube showed a thick creamy 

 layer, perhaps slightly less in No. 4 (boiled extract), but still very obvious. All 

 four showed, iinder the microscope, fields crowded with oil globules of all sizes. 



