142 



Prof. B. Moore. On the Synthesis of [June 15, 



Pancreas. 



This series of tubes, when examined 1 hour after the commencement of the 

 experiment, showed in each case a milky fluid, and the microscopic examination 

 demonstrated abundance of oil globules. 



The reaction was obviously more intense than in the case of the intestine. 



After the lapse of 18 hours from the commencement of the experiment, when it 

 was stopped to make the analyses detailed below, there was a thick creamy layer at 

 the top of each tube which was not apparently any less in quantity in No. 4 (boiled 

 extract) than in any of the others. 



The microscope showed in all four tubes oil globules of all sizes in great number. 



The eight tubes were extracted with ether, and the weights of the ethereal 

 extracts,* and the percentages of free oleic acid they contained, are given in the 

 following table : 



This experiment shows that with cell-free extracts of pancreas and intestinal 

 mucosa the main effect obtained is a conversion of sodium oleate into free oleic 

 acid, and that there is no appreciable formation of olein. 



Expt. 5. Control Experiment. It might be argued that in the preceding experi- 

 ments the soap was hydrolysed by the water or saline used as a solvent, and not by 

 any active constituent derived from the tissues extracted, and accordingly a series 

 of controls were arranged in which the same percentage of 'soap was dissolved, as 

 follows : 



No. 1. Distilled water, 40 c.c. + sodium oleate, 0'8 gramme + glycerine, 0'4 



gramme. 

 No. 2. Normal saline (075 per cent.), 40 c.c. + sodium oleate, 0'8 gramme 



+ glycerine, 0'4 gramme. 

 No. 3. Solution of normal sodium carbonate (0'2 percent.), 40 c.c. -f saturation 



with carbon dioxide + glycerine, 0'4 gramme. 

 No. 4. Oxalated pig's plasma, 40 c.c. + sodium oleate, 0'8 gramme + glycerine, 



0'4 gramme. 



* The weighings throughout these experiments have been taken to 

 gramme. The figures represent fractions of a gramme. 



milli- 



