144 Prof. B. Moore. On the Synthesis of 



The results are given in the following table : 



[June 15, 



The free oleic acid obtained in No. 2 is derived from the neutral fat of the 

 intestinal mucosa and not from the sodium oleate (compare Experiment 1, Series C). 

 The difference between free oleic acid in No. 1 and No. 2 is due to hydrolysis of 

 the sodium oleate in No. 1, and it is evident from the differences in column 3 that 

 there is no appreciable synthesis of neutral fat. In fact, the difference lies in the 

 opposite direction in Experiment 2 of this series (vide infra), proving that the 

 0-0693 gramme here apparently formed (0-1891 01198 gramme) lies within the 

 limit of experimental error. 



Expt. 2. The abdominal lymphatics of the animal used in Experiment 1 were 

 finely divided, and treated in similar fashion to the intestinal mucosa used in that 

 experiment. 



Portion No. 1 weighed 1'82 grammes, and to this were added 18 c.c. of normal 

 saline, 0'36 gramme of sodium oleate, and 0'15 gramme of glycerine. 



Portion No. 2 weighed T63 grammes, and to this were added 16 c.c. of normal 

 saline only as a control. The two mixtures were digested at 36 C. for an interval of 

 115 hours, both were then evaporated to dryness, and 0'32 gramme of sodium oleate 

 was added to No. 2. Each was then extracted four times with ether, the united 

 ethereal extracts in each case were evaporated to dryness, and the weights of total 

 ethereal extract and amounts of free oleic acid were determined. 



The results were for ease of comparison calculated to 2 grammes of tissue, and 

 are given in the following table : 



Here the difference between total extract and free oleic acid is greater in the case 

 of the control, showing that there is no synthesis of neutral fat. 



Series C. 



In this series of experiments, in addition to determining the amount 

 of free oleic acid in the ethereal extract, the amount of neutral fat was 



* The lower percentage of oleic acid obtained in this and in the preceding 

 experiment is due to the dry method of extraction, in which more soap is dissolved 

 out than in the wet extraction employed in Series A ; the difference is not due to 

 neutral fat, but to soap, as is conclusively shown by the experiments in Series C. 



