1903.] Action of Choline, Neurine, Muscartne, and Betaine. 333 



Experiment I. Betaine Hydrochloride. 2 per cent, solution. 



FIRST NERVE. 



In 1/10000 of a volt. 



Current of Negative 



injury. variation. 



After soaking in saline for 2 hours + 25 4 



After new transverse section + off scale 15 



After further 12 hours in saline +25 19 



After new transverse section + off scale 22 



After soaking in beta'ine for 20 niins +32 12 



After further 3 hours in betaine +33 



After further 9 hours in saline + off scale 27 



After further 10 hours in saline + off scale 11 



After interpolar crush 



SECOND NERVE. 



After soaking in saline for 1 hour +18 4 



After new transverse section + off scale 25 



After soaking in betaine for 1 hour +12 



After new transverse section +50 



* After soaking in saline for 12 hours 2 + 6 



* After new transverse section + off scale 33 



After 1 hour in betaine +50 



After new transverse section + off scale 



After 12 hours in saline 11 



After new transverse section 17 



Betaine, as regards its direct effect upon nerve, is a substance of the 

 second class, as defined above, viz., moderately active. This conclusion 

 is borne out by the similar results of further trials at higher and lower 

 strengths of solution. 



Exp. II. Betaine. 

 FIRST NERVE. 



Current of 

 injury. ^ e g var 



hours. In saline for 1 hour + 25 - 5 



1 + 22 4 



New transverse section + off scale -15 



12 In saline for 12 hours +25 -19 



New transverse section + off - 22 



12i In bet. hyd., 2 p. c. for 25 mins. ... + 32 - 12 



16 In bet. hyd. for 3J hours +33 



25 In saline for 9 hours + off -27 



35 Ditto for 10 hours + off -11 



Then interpolar crush , 



* Plate 3503. 



