362 Dr. A. E. Wright and Capt. S. R. Douglas. [Sept. 1, 



DOES THE SUBSTITUTION OF ANOTHER MEDIUM FOR THE (CITRATED) 

 BLOOD PLASMA WHICH BATHES THE CORPUSCLES EXERT AN 

 INFLUENCE ON PHAGOCYTOSIS 1 



1. Comparative Experiments with Citrated Plasma and Citrated Strum 

 (obtained Respectively as Described in Connection with Procedures 2 and 3). 



EXPERIMENT 1. 



A. S. R. D.'s plasma, 3 vols. ; staphylococcus suspension, 1 vol. ; 

 A. E. W.'s corpuscles, 3 vols. 



Tube 1. Phagocytic power (obtained by averaging the 

 number of staphylococci ingested by 20 

 P.W.B.C.) 34 ' 6 \34-l 



Tube 2. Do. do. do. 33-6.J 



B. S. R. D.'s sei-um, 3 vols. ; staphylococcus suspension, 1 vol. ; 

 A. E. W.'s corpuscles, 3 vols. 



Tube 1. Phagocytic power (elicited as above) ... 35 6 "1 ~. . * 

 Tube 2. Do. do. 33- 8 J 



EXPERIMENT 2. 



A. A. E. W.'s plasma, 3 vols. ; staphylococcus suspension, 1 vol. ; 

 A. E. W.'s corpuscles, 3 vols. 



Tube 1. Phagocytic power (elicited as above) ... 31 -2 "I o c 

 Tube 2. Do. do. ... 36-0 J 



B. A. E. W.'s serum, 3 vols. ; staphylococcus suspension, 1 vol. ; 

 A. E. W.'s corpuscles, 3 vols. 



Tube 1. Phagocytic power (elicited as above) ... 31 *2 1 o. 2 i 

 Tube 2. Do. do. ... 33-0 J 



It is clear that the phagocytic power is uninfluenced by the sub- 

 stitution of serum for plasma. 



2. Comparative Experiments with Ordinary (Uncitrated) Serum Unheated 

 and Heated f oi' 10 15 min. to 60 65 C. and tlien cooled. 



EXPERIMENT 1. 



A. A. E. W.'s unheated serum, 3 vols. ; staphylococcus suspension, 

 1 vol. ; A. E. W.'s corpuscles, 3 vols. 



Tube 1. Phagocytic power (bacteria in 20 P.W.B.C. 



enumerated and averaged) 1 7 4 



Tula 2. Do. do. 19 vs 



