1903.] Role of the Blood Fluids in Phagocytosis. 365 



Do THE BLOOD FLUIDS CO-OPERATE IN PHAGOCYTOSIS BY EXERTING 

 A DIRECT " STIMULATING " EFFECT UPON THE PHAGOCYTES, OR BY 

 EFFECTING A MODIFICATION IN THE BACTERIA 1 



The following experiments were instituted with a view to elucidating 

 the problem as to the nature of the activating influence exercised by 

 the blood fluids. It will be seen that- a comparison is in each case 

 instituted between serum inactivated (by heating) before it came in 

 contact with either bacteria or white corpuscles, and serum inactivated 

 after it had come in contact with the bacteria, but before it had come 

 in contact with the white corpuscles : 



EXPERIMENT 1. 



A. S. R. D.'s inactivated serum, 3 vols. ; staphylococcus suspension 

 (previously heated to 60 C. for 15 minutes and cooled), 1 vol. ; 

 S. R. D.'s corpuscles, 3 vols. 



Tube 1. Phagocytic power (bacteria in 20 P.W.B.C. 



counted and averaged) 3*4 



Tube 2. Do. do. 3*35 



B. S. R. D.'s unheated serum, 3 vols. ; digested at 37 C. for 

 15 minutes, with 1 vol. of staphylococcus suspension, then heated to 

 60 C. for 15 minutes and cooled. 



4 vols. of the above mixed with 3 vols. of S. R. D.'s corpuscles. 



1. Phagocytic power (bacteria in 20 P.W.B.C. 



counted and averaged) 27*5 



. Do. do. 28-9 



EXPERIMENT 2. 



A. A. E. W.'s inactivated serum, 3 vols. ; staphylococcus suspen- 

 sion, 1 vol. ; digested together for 15 minutes at 37 C., then heated for 

 10' minutes to 60 C. and cooled. 



4 vols. of the above mixed with 3 vols. of S. R. D.'s corpuscles. 



Tube 1. Phagocytic power (bacteria in 20 P.W.B.C. 



counted and averaged) 4'0 



Tube 2. Do. do. 3-2 



B. A. E. W.'s unheated serum, 3 vols. ; staphylococcus suspension, 

 1 vol. ; digested together for 15 minutes at 37 C., then heated for 

 10 minutes to 60 C. and cooled. 



4 vols. of the above added to 3 vols. of S. R. D.'s corpuscles. 



Tube 1. Phagocytic power (bacteria in 24 P.W.B.C. 



counted and averaged) 33 



Tube 2. Phagocytic power (bacteria in 19 P.W.B.C. 



counted and averaged) 36 



