180 



PRINCIPLES OF BACTERIOLOGY 



TABLE III. 



Serum from Two Horses at the End of One Year, during which Weekly Injections were Given. 



Dysentery B. (Shiga). Paradysentery (Manila). 



Culture. l': 500 1 : 1000 1 : 5000 iTsOO 1 : 1000 1 : 5000 



Dysentery B., Japan + + + 



Paradysentery B., Mt. Desert + + + 



" Manila. ..+++ + -t- + 



It is interesting to note that the serum from the horse receiving the 

 Shiga culture agglutinates the paradysentery culture in higher dilutions 

 .than the Shiga culture. In the other serum all cultures were agglu- 

 tinated in equally high dilutions in spite of the fact that the paradysen- 

 tery type had been injected. 



THE RELATIVE ACCUMULATION OF THE GROUP AND SPECIFIC AGGLU- 

 TININS. This is seen to vary for the different types and at different 

 times. For the Manila culture of Flexner, which is nearest to the colon 

 in its characteristics, the specific agglutinins were in the serum of an 

 animal which had received injections of the Manila cultures at the 

 end of the third month six times as abundant as the group agglu- 

 tinin acting on the Maine culture of Park, which represents a type 

 farther removed from the colon. At the end of the fourth month they 

 were fourteen times as abundant. For the dysentery bacillus (Shiga) 

 the development of agglutinins was the least. 



Another point of interest is that the proportional amount of agglu- 

 tinins from the different cultures varied at different times. If on tests 

 made of a single bleeding we had attempted to draw conclusions as to 

 the relative development of specific and group agglutinins between 

 the cultures, we would have had an imperfect view. Many conflicting 

 statements in literature are undoubtedly due to this lack of apprecia- 

 tion of the variability in the relative amount of these two types of agglu- 

 tinins during a long process of immunization. 



The development of group agglutinins for the three dysentery types 

 caused by the injections of a colon bacillus : 



1st 



2d 



FIG. 70 



3d 4th 



5th 



6th* 



7th 



The rise and fall of common and specific agglutinins during seven months in a rabbit 



injected with the Manila culture after it had been heated to 115 C. 

 Colon bacillus X. 



Paradysentery type (Manila). 



Paradysentery type (Mt. Desert). Dysentery type (Japan). 



Test dates for all four sera. 



* Injections stopped. 



