260 



BACTERIA PATHOGENIC TO MAN 



TABLE III. Showing agglutinations of members of three types in the serum of animals 

 injected with bacilli of Type III. 



Rabbit injected with Baltimore, Duval. 



To 50 100 500 1000 5000 10,000 



Type I. 

 1. Japan Shiga, and 5 other cultures, ++ + + 



Typell. 

 6. Mt. Desert Park, and 5 other cultures, + + + + + 



Type III. 

 Manila Flexner, and 5 other cultures, ++ ++ + + + + ++ ++ + 



Previous to immunization the serum agglutinated the bacilli of Type III. in 1 : 20 dilution, but 

 none of the others even in 1 : 10. This is one of the few animals in which agglutinins for Type I. 

 developed through the injections of bacilli of the other types. 



TABLE IV. Showing how Type III. is unable to absorb the agglutinins produced through 

 injections of Type II. Serum from rabbit inoculated with Mt. Vernon culture, Type II. 



Agglutinins exhausted with 



Type I. 

 Shiga, 5 other cultures, 



Type II. 



Mt. Desert, 5 other cultures, 

 Type III. 



Manila, 5 other cultures, 



Serum 

 before 

 absorp- 

 tion. 



Baltimore, Duval. 



1 : 20 1 : 50 1 : 100 1 : 200 1 : 400 



Mt. Vernon, c.c. a 

 T: 20 1 : 50 1 : 100 



1:10 



1:600 ++ ++ 



1 : 100 



+ + 



Before injections this rabbit's serum agglutinated Types II. and III. 

 in 1:20 dilutions. 



The considerable amount of common agglutinins affecting Type II. 

 and Type III. is seen to be absorbed by the bacilli of either type. 

 The larger amount of specific agglutinin is left in the serum when any 

 bacillus other than one of identical characteristics with the bacillus 

 used in the immunization is employed. 



TABLE V. Showing that horse injected with Shiga and Philippine types develop specific 

 agglutinins for the bacilli belonging to these two types and common agglutinins for the 

 .varieties included under Type II. 



Cultures. 



Type I. 



Shiga, original, and 4 others, 

 'Type II. 



Park, original, and 4 others, 

 Type II. (B.) 



Brooklyn, 

 Type II. (C.) 

 Type II. (D.) 



Type III. 

 Flexner original and 4 others, 



Serum 

 after 

 injec- 

 tions for 

 several 

 months. 



+600 



+600 

 +300 

 +600 



1200 



Same serum after saturation with cultures of 



Shiga 

 Type. 



Type III. Type II. 



Pyocy- 

 aneus. 



Typhoid. Colon. 



+1500 10 



10 



+20 

 10 

 20 



+400 

 10 



+10 

 10 

 10 



+700 +1000 



10 



+50 

 +50 

 +50 



+600 



+800 



+50 



+100 



+400 10 



+500 +800 



+300 

 +30 



+100 



+10 



+30 



+300 



+300 

 +50 



+50 

 +20 

 +60 



+600 



The manipulation necessary in making dilutions and filtering, as well 

 as the effect of standing, cause a certain amount of destruction of 

 agglutinins. 



