AM(EBINA 481 



until no more blue stain comes from the specimen, then stain with 1 

 per cent, eosin. The nuclei of the leukocytes are stained blue, while 

 those of the amoebae are stained red. 



Mallory and Wright recommended the following method for sections 

 containing amceba coli: 1. Harden in alcohol. 2. Stain sections in a 

 saturated aqueous solution of thionin three to five minutes. 3. Differ- 

 entiate in a 2 per cent, aqueous solution of oxalic acid for one-half to 

 one minute. 4. Wash in water. 5. Dehydrate in alcohol. 6. Clear 

 in oleum origani cretici. 7. Wash off with xylol. 8. Xylol balsam. 



Amoebae in Diseases Other than Dysentery. Kartulis reported finding 

 a large motile amoeba (30/* to 38,) in an abscess of the lower jaw of an 

 Arabian. Flexner also observed a similar case in a sixty-two-year-old 

 man. Baelz found a very large amoeba in the bloody urine and in the 

 vagina of a twenty-three-year-old Japanese who was suffering from 

 tuberculosis of the lung. Jiirgens, Kartulis, and Posner also reported 

 finding similar amoebae in cases of cystitis and bloody urine. 



In the ascitic fluid of a man who had carcinoma of the stomach 

 Leyden found motile cellular elements which Schaudinn pronounced 

 independent organisms belonging to the rhizopoda Leydenia gem- 

 mipara (Schaudinn), Fig. 145. Similar organisms were found in the 

 ascitic fluid of a girl who had an abdominal tumor. The organisms 

 remained motile in the ascitic fluid seven days after its removal. The 

 organism possesses a pulsating vacuole and one vesicular nucleus; it 

 divides directly and by budding. The individuals seem readily to fuse 

 (plastogamy). The pathological significance of this rhizopod is not 

 clear. 



31 



