494 PROTOZOA 



Case I. Male, aged twenty years. Luetic infection December, 1904. 

 No regular treatment. He presented mucous patches of the tonsils 

 and soft palate, and a fading rash of the trunk. Between the buttocks, 

 flat condylomata. A condyloma was excised. Smears were made and 

 stained in various anilines and with eosin-azur. Fresh preparations in 

 salt solution were also studied. In the latter no characteristic organ- 

 isms were found. The stained preparations were positive, showing a 

 variable number of thin, lightly stained spiral organisms identified 

 as the species described by Schaudinn and Hoffmann. The positive 

 results were obtained with aniline-water-gentian violet and eosin-azur, 

 the latter having given the most satisfactory results. The films varied 

 greatly, even with the same method of preparation. In some the num- 

 ber of stained spirals was very small, while in others the number was 

 large, a single field showing as many as five. In still other cover- 

 glasses no organisms could be discovered. The spirals were long for 

 the most part and showed from six to twelve bends or curves. 



Case II. Male, aged thirty-four years. Burrowing non-syphilitic 

 ulcer of the penis. Smears from the surface only could be obtained, 

 and they were negative for spiral micro-organisms. 



Case III. Male, aged twenty-three years. Mucous patches in mouth. 

 Healed scar on penis. Enlargement and induration of glands of the 

 groin. A small quantity of fluid, consisting of blood and lymph cells 

 aspired from the enlarged glands. Fresh preparations negative; smears 

 stained in eosin-azur showed a very few delicate, faintly-staining spiral 

 organisms. 



Case IV. Male, aged eighteen years. Infection in January. For 

 several weeks active antisyphilitic treatment. Primary lesion on glans 

 penis; marked swelling of the glands of the groin. Part of primary 

 lesion was excised and fluid was aspirated from an enlarged gland. 

 In the fresh fluid of the gland diluted in sterile salt solution several 

 small and a single larger motile spiral organism were seen. None of 

 the stained preparations from the primary lesion or the gland juice 

 showed spirochsetes. 



^ Case V. Colored male, aged twenty years. No definite history of 

 time of infection. Presented himself with phimosis and balanitis. 

 After incision of the prepuce three separate elevated, indurated lesions 

 regarded as chancres appeared. Moderate swelling of the glands of the 

 groin. One of the lesions excised. Smears and fresh preparations 

 were made from the base of the lesion. Fresh preparations negative. 

 The smears stained in eosin-azur showed a moderate number of delicate 

 spirals, agreeing with the description given by Schaudinn and Hoff- 

 mann. They varied in number in different cover-glasses, and in some 

 could not be found. The curves numbered from eight to twelve. 



Case VI. Male, aged twenty-three years. Non-syphilitic ulcer of 

 penis; duration twelve days. Films stained in eosin-azur were nega- 

 tive for spirochsetes. 



Of the four cases of syphilitic lesions studied by Flexner and Noguchi 

 the spiral organisms were obtained in stained preparations three times, 



