32 ESSENTIALS OF BACTERIOLOGY 



FORMULAS OF DIFFERENT STAINING SOLUTIONS 

 I. Saturated Alcoholic Solution. 



Place about 10 grams of the powdered dye in a bottle and 

 add 40 grams of alcohol. Shake well and allow to settle. 

 This can be used as the stock bottle. 



II. Weak Solutions. 



Made by adding about i part of stock solution (I) to 10 

 parts of distilled water. This is the ordinary solution in use. 



III. Anilin-oil Water. 



Anilin-oil 5 parts. 



Distilled water 100 " M. 



Shake well and filter. To be made fresh each time. 



IV. Anilin-water Dyes. 



Saturated alcoholic solution of the dye n parts. 



Anilin-oil water 100 



Absolute alcohol 10 " M. 



Can be kept ten days. 



V. Alkaline Methylene-blue. 



A. Loffler's: 



Saturated alcoholic solution methylene- 



blue 3- 



Solution potassium hydroxid (i : 10,000) 100.0. M. 



B. Koch's: 



Solution potassium hydroxid (10 per 

 cent.) 0.2 



Saturated alcoholic solution methylene- 

 blue i.o 



Distilled water . . 200.0. M. 



