STAINING OF BACTERIA 33 



VI. Phenol Solutions. 



A. Ziehl-Neelsen: 



Fuchsin (powdered) i part. 



Alcohol 10 parts. 



5 per cent, solution phenol 100 " M. 



Filter. The older the solution, the better. 



B. Kiihne: 



Methylene-blue 1.5 



Alcohol 10.0 



5 per cent, solution phenol 100.0 



Add the acid gradually. This solution loses strength with 

 age. 



VII. Gram's lodin Solution. 



lodin i.o 



Potassium iodid ! 2.0 



Distilled water 300.0 M. 



VIII. Loffler's Mordant (for Flagella). 



Aqueous solution of tannin (20 per 



cent.) ....*. 10 parts. 



Aqueous solution ferric sulphate (5 per 



cent.) - i part. 



Aqueous decoction of logwood (i : 8) . . 4 parts. M. 

 Keep in well-corked bottle. 



IX. llnna's Borax Methyl-blue. 



Borax i part. 



Methyl blue i part. 



Water 100 parts. M. 



X. Gobbet's Acid Blue (Rapid Stain). 



Methylene-blue 2.0 



25 per cent, sulphuric acid 100.0 M. 



3 



