34 ESSENTIALS OF BACTERIOLOGY 



XI. Alkaline Anilin-water Solutions. 



Sodium hydroxid (i per cent.) i.o 



Anilin-oil water 100.0 M. 



And add 



Fuchsin, or methyl- violet powdered . . . 4.0 



Cork well. Filter before using. 



XII. Roux's Double Stain. 



Dahlia or gentian-violet 0.5 gm. 



Methyl-green 1.5 " 



Distilled water 200.0 " M. 



Use as other stains, without acid. 



XIII. Neisser's Stain (jor Diphtheria). 



Solution I. 



Methylene-blue i gm. 



Alcohol (96 per cent.) 20 c.c. 



Dissolve and add 



Water 950 c.c. 



Glacial acetic acid 50 c.c. M. 



Solution II. 



Vesuvin 2 gm. 



Water 1000 c.c. M. 



Stain cover-glasses (i) Three seconds in solution I; (2) 

 wash in water; (3) three seconds in No. 2; (4) wash in water. 

 Body of bacillus, brown; oval granules at each end, blue. 



XIV. Carbol-thionin (Nicolle). 



Saturated solution thionin in alcohol (90 



per cent.) 10 c.c. 



Aqueous solution phenol (i per cent.) . . 100 c.c. M. 

 Stain sections one-half to one minute. 



