SPECIAL METHODS OF STAINING AND MODIFICATIONS 43 



Weigert uses anilin-oil to dehydrate instead of alcohol, and 

 here, too, it can be used mixed with the dye. 



Capsule Stain (Buerger). I. Spread culture by means of a 

 drop of ascitic fluid on cover-glass. 



II. Fix in Miiller's fluid, which has been saturated with 5 

 per cent, bichlorid of mercury, and warm for three seconds. 



III. Wash quickly in water; rinse in alcohol. 



IV. Cover with tincture of iodin for one minute. 



V. Wash in alcohol and dry in air. 



VI. Stain in anilin-water gentian-violet for two seconds. 



VII. Wash in 2 per cent, salt solution. 



VIII. Mount in salt solution ringed with vaselin. 



Flagella Stain, with Lo frier's Mordant. I. A few drops 

 of the mordant (No. VIII, p. 33) are placed upon the spread 

 cover-glass and heated until it steams. 



II. Wash with water until the cover-glass looks almost clean, 

 using a small piece of filter-paper to rub off the crusts which 

 have gathered around the edges. 



III. Anilin-water fuchsin (neutral) held in flame about one 

 and one-half minutes. 



IV. Wash in water. 



If the stain is properly made, the bacteria are deeply colored 

 and the flagella seen as little dark lines attached to them. 



Unna's Method for Fungi. Especially useful for epidermic 

 scales. Moisten horny scale or crust with acetic acid; mace- 

 rate between two glass slides; dry in flame; wash out fat with 

 ether and alcohol (equal parts) ; stain in borax methyl blue for 

 ten seconds (over flame) ; bleach with glycerin and ether (equal 

 parts) ; rinse in water, alcohol, dry and mount. 



