SOLID TRANSPARENT MEDIA 53 



with distilled water until thick, and then sterilized in flasks 

 three-quarters of an hour for three successive days. 



Fig. 22. Method of inoculation (Woodhead and Hare). 



Bread Mash. Bread devoid of crust, dried in an oven, and 

 then pulverized and mixed with water until thick, and sterilized 

 as above. 



CHAPTER IX 

 SOLID TRANSPARENT MEDIA 



Solid transparent media are prepared from materials which 

 can be used for microscopic purposes and which can readily be 

 converted into liquids. Such are the gelatin and agar culture- 

 media. 



Gelatin. Gelatin is obtained from bones and tendons, and 

 consists chiefly of chondrin and gluten. 



The French Golden Medal brand is the one most in use, 

 found in long leaves with ribbed lines crossing them. 



Koch-Lbffler 10 per cent. Bouillon-gelatin. To the 

 meat-water as made for the bouillon are added 100 grams 

 gelatin, 10 grams peptone, 5 grams salt, to each 1000 grams 



