1OO ESSENTIALS OF BACTERIOLOGY 



Growth. Somewhat quickly; facultative anaerobic; growth 

 only at ordinary temperatures. 



Plates. White colonies, with greenish-yellow fluorescence 

 around each colony. Under microscope the periphery appears 

 radiated. 



Stab-cultures. Good growth along the needle-thrust; the 

 whole gelatin gives out the fluorescence. 



Bacillus Fluorescens Liquefaciens. Origin. Water and 

 from conjunctival sac. 



Form. Very fine little rods; no spores. 



Properties. Motile; forms a greenish-yellow, fluorescent pig- 

 ment; liquefies gelatin. 



Growth. Rapid at ordinary temperature and strongly aerobic. 



Plates. Round colonies, cup-shaped depressions, the solid 

 gelatin that remains becoming colored with greenish-gellow 

 fluorescence. 



Stab-culture. On the surface, air-bubble depressions; the 

 white colonies in the bottom of these depressions and the solid 

 gelatin around the inoculation shining with the fluorescence. 



Phosphorescent Bacteria. Are found usually in sea-water 

 or upon objects living in the sea. 



Bacillus Phosphorescens Indicus (Fischer). Origin. 

 Tropical waters. 



Form. Thick rods, with rounded ends, sometimes forming 

 long threads. 



Properties. Very motile; liquefying gelatin at a tempera- 

 ture of 25 to 30 C., with oxygen and a little moisture, and in 

 the dark, a peculiar electric-blue phosphorescence develops. 



Growth. Slowly; must have oxygen; does not grow under 

 10 C. or over 50 C. 



Plates. Little round, gray points, which under low power 

 appear as green colonies with reddish tinge around them. 

 Cooked fish, when smeared upon the surface with a little of the 

 culture, show the phosphorescence most marked. Grows well 

 on potatoes and blood-serum. 



Bacillus Phosphorescens Indigenus (Fischer)^. Origin. 

 Waters in the northern part of Germany. It differs from the 



