l6o ESSENTIALS OF BACTERIOLOGY 



onies, like strings of beads, which after a time become one solid 

 white string. 



Stroke-culture on Agar. Little drops, never coalescing, hav- 

 ing a bluish tint, very transparent. 



Potato. No apparent growth. 



Bouillon. At 37 C. clouds are formed in the bouillon, 

 which' then sink to the bottom, and long chains of cocci found 

 in this growth. 



Staining. Easily colored with the ordinary stains. Gram's 

 method is also applicable. 



Patho genesis. Inoculated subcutaneously in the ear of a 

 rabbit, an erysipelatous condition develops in a few days, 

 rapidly spreading from point of infection. 



In man, inoculations have been made to produce an effect 

 upon carcinomatous growths and erysipelas was always pro- 

 duced. When it occurs upon the valves of the heart, endo- 

 carditis results. Puerperal fever is caused by the microbe 

 infecting the endometrium, the Streptococcus puerperalis of 

 Frankel being the same germ. 



In scarlatina, variola, yellow fever, cerebrospinal meningitis, 

 and many similar diseases, the microbe has been an almost con- 

 stant attendant. It is often associated with the diphtheria 

 bacillus in true diphtheria, and is the cause of many of the 

 diphtheritic affections of the throat in which the diphtheria 

 bacillus is absent. 



An antistreptococcic serum has been used as a curative 

 agent in puerperal fever, scarlatina, and other diseases sup- 

 posed to be due to this germ. 



A mixture of a culture of pyogenes and prodigiosus has been 

 used as an injection, with apparent benefit, in inoperable cases 

 of sarcoma, and is known as Coley's fluid. 



Staphylococcus Pyogenes Aureus (Rosenbach) . Origin. 

 Found commonly in pus (80 per cent, of all suppura- 

 tions), in air, water, and earth; also in sputum of healthy 

 persons. 



Form. Micrococci in clusters like bunched grapes, hence the 

 name staphylo, which means grape. They never form chains. 



