1 66 ESSENTIALS OF BACTERIOLOGY 



Stains. With carbol-fuchsin, and is often intracellular. 

 Does not take Gram. 



Patho genesis. Very contagious, found in 10 per cent, to 20 

 per cent, of all cases of conjunctivitis. Not infectious for lower 

 animals, and not causing any other form of disease. 



Morax-Axenfeld Diplobacillus of Conjunctivitis. 

 This bacillus is found in the greater number of cases of con- 

 junctivitis. 



Form. A short, plump bacillus, usually in pairs and chains 

 of pairs. Non-motile (Fig. 100). 



Growth. With difficulty in blood-serum agar, it forms small 

 pitted colonies or lacunae ; liquefies. 



Staining. Does not take Gram, but stains readily. 

 Non-pathogenic for lower animals. 



Micrococcus Gonorrhoeas (Gonococcus) (Neisser). In 



1879 Neisser demonstrated the presence of 



this germ in the secretion of specific urethritis. 



, Form. Cocci, somewhat triangular in 



form, found nearly always in pairs, the base 



of one coccus facing the base of the other 



f v . and giving the appearance of a Vienna roll, 



hence the German name, semmel (roll), form. 



Four to twelve such pairs are often found 



together. Immotile (Fig. 102). 



Gon F oc g occi ingo^ Culture. On gelatin-agar or potato they 

 orrheaipus. Ani- d o not grow, and onlv upon human blood- 



lin methyl-violet ' . ( 



(x 650). serum have they given any semblance of a 



growth. The temperature must be be- 

 tween 33 and 37 C., and the growth occurs very slowly and 

 sparsely. 



Method of Cultivation (Wertheini) . Gonorrheal pus is mixed 

 in a test- tube with liquid human blood-serum of 40 C. temper- 

 ature, and two dilutions are made with blood of the same tem- 

 perature. An equal quantity of 2 per cent, agar solution is 

 now poured into each tube, and three glass dishes are covered 

 at once with this mixture. After being in the brood-oven for 

 twenty-four hours, colonies can be discovered. 



