PATHOGENIC BACTERIA 167 



In three days a very thin, almost invisible, moist yellowish 

 growth, seemingly composed of little drops. 



Under low power small processes are seen shooting out from 

 the smooth border. 



It requires then to be transferred to fresh media, as the cul- 

 ture quickly perishes. 



Cultivation has also occurred on acid gelatin, gelatin con- 

 taining acid urine, in acid urine itself, and albuminous urine 

 with agar. 



Fig. 102. Gonococcus in urethral pus (x 1000) (Frankel and Pfeiffer). 



In septicemic cases the gonococcus has been isolated from 

 the blood direct by drawing 5 to 10 c.c. from a vein and adding 

 it in equal parts to melted agar. The mixture is poured into 

 Petri dishes and developed in the incubator at 37 C. 



Staining. Colored easily with all ordinary anilin stains. 



Gram's method is not applicable, this being one of its main 

 diagnostic features. 



The following method is recommended by Neisser. 



The cover-glasses, with some of the urethral discharge 

 smeared upon them, are covered with a few drops of alcoholic 



