214 



ESSENTIALS OF BACTERIOLOGY 



The new tissue, composed of round-cells, then undergoes 

 softening, purulent collections form, and the normal structure 

 is destroyed. 



The usual seat is in the maxillary bones, but the fungus has 

 been found in the lungs, tonsils, intestines, and various other 



,_ organs in man and cattle. 



Examination. Well seen 

 in the unstained condition. 

 jjjjj From the pus or scraping a 

 small portion is taken and 

 squeezed upon the glass slide; 

 IJH if calcareous matter is pres- 

 js|| ent, a drop of nitric acid will 

 dissolve the same. 



Glycerin will preserve the 

 preparation. 



Staining. Cover -glass 

 specimens stained best with 

 Gram's method. Tissue sec- 

 tions should be stained as 

 follows : 



Ziehl's carbol-fuchsin, ten 

 minutes. Rinse in water. 



Concentrated alcoholic 

 solution of picric acid, five 

 minutes. Rinse in water. 



Alcohol, 50 per cent., fif- 

 teen minutes. Alcohol abso- 

 lute, clove-oil, balsam. 

 The rays stained red, the tissue yellow. 

 Streptothrix Madurae (Vincent). Origin. Found in the 

 disease known as Madura foot, or mycetoma, an ulceration 

 affecting the feet, especially of individuals living in the tropics. 

 Two varieties, the pale and the black, have been described. 



Form. Branched filaments resembling the actinomyces 

 Streptothrix in the mycelia. Spores are seen. 

 Cultivation. In liquid media containing vegetable infusions 



Fig. 130. Streptothrix Madurae in a 

 section of diseased tissue (Vincent). 



