MOUNTING AND PREPARATION OF OBJECTS. 71 



tubercle bacillus in sputa or diseased tissue, and is 

 an abstract from a paper read in the pathological 

 section of the British Medical Association at Wor- 

 cester, in August, 1882, by Dr. Heneage Gibbes. 



The following stock solutions are prepared : 



I. Magenta Crystals, 2 grammes 

 Pure Aniline, 3 c.c. 

 Alcohol, sp. gr. -830, 20 c.c. 

 Distilled Water, 20 c.c. 



Dissolve the aniline in the spirit; rub up the 

 magenta in a glass mortar ; add the spirit gradually 

 while stirring, till all the colour is dissolved, then 

 add the water slowly, still stirring, and put in a 

 stoppered bottle. 



II. Saturated solution of chrysoidin in dis- 

 tilled water, to which is added a crystal of 

 thymol dissolved in a little absolute alcohol. 



This solution must be kept in a dark place. 



III. Commercial Nitric Acid, 1 part. 

 Distilled Water, 2 parts. 



The first two solutions must not be filtered. 



The solutions are used as follows : 



The sputum is spread on a cover glass and al- 

 lowed to dry, it is then passed three or four times 

 through the flame of a spirit lamp, and left to cool. 

 A few drops of solution number 1, are filtered into 

 a watch glass, and the cover glass, sputum down- 

 wards, placed on it, care being taken that no air 



