METHODS OF STUDYING BACTERIA 51 



B. Kiihne: 



Methylene-blue 1.5 parts 



Alcohol 10.0 " 



5 per cent, solution phenol 100.0 " 



Add the phenol gradually. This solution loses strength 

 with age. 



VII. Gram's lodin Solution 



lodin i part 



Potassium iodid 2 parts 



Distilled water 300 " M. 



VIII. Lqffler's Mordant (for Flagella) 

 Aqueous solution of tannin (20 per 



cent.) 10 parts 



Aqueous solution ferric sulphate (5 



per cent.) i part 



Aqueous decoction of logwood (i : 8) 4 parts. M. 

 Keep in well-corked bottle. 



IX. Unnd's Borax Methyl-blue 



Borax i part 



Methyl blue i " 



Water 100 parts. M. 



X. Gabbet's Acid Blue (Rapid Stain) 

 Methylene-blue 2 parts 



20 per cent, sulphuric acid ". 100 " M. 



XI. Alkaline Anilin-water Solutions 

 Sodium hydroxid (i per cent.) .... i part 

 Anilin-oil water 100 parts. M. 



And add 



Fuchsin, or methyl-violet powdered 4 parts 

 Cork well. Filter before using. 



