52 ESSENTIALS OF BACTERIOLOGY 



XII. Roux's Double Stain 



Dahlia or gentian- violet 0.5 part 



Methyl-green 1.5 parts 



Distilled water 200.0 " M. 



Use as other stains, without acid. 



XIII. Neisser's Stain (for Diphtheria) 

 Solution I 



Methylene-blue i part 



Alcohol (96 per cent.) 20 parts 



Dissolve and add 



Water 950 parts 



Glacial acetic acid 50 M. 



Solution II 



Vesuvin 2 parts 



Water 1000 " M. 



Stain cover-glasses (i) Three seconds in solution I; (2) 

 wash in water; (3) three seconds in No. II; (4) wash in water. 

 Body of bacillus, brown; oval granules at each end, blue. 



XIV. Carbolthionin (Nicolle) 

 Saturated solution thionin in alcohol 



(90 per cent.) 10 parts 



Aqueous solution phenol (i per 



cent.) TOO " M. 



Stain sections one-half to one minute. 



XV. Capsule Stain of Hiss 

 Use the following, heated until it 

 steams: Saturated alcoholic solu- 

 tion of gentian-violet or f uchsin . . 5 parts 



Distilled water 95 " M. 



Wash in 20 per cent, solution of cupric sulphate crystals. 



