ANAEROBIC BACTERIA l8l 



Bouillon. Adding glucose to the bouillon gives a medium 

 in which an abundant growth occurs. 



Stab-agar. Inverted fir-tree appearance. 



Milk. Acid reaction and slow coagulation. 



Inoculation of animals with suspected material may be 

 necessary as preliminary step. 



Cultivation from Spores. Kitasato, by exposing a portion 

 of suspected material to a temperature of 80 C. for one hour, 



Fig. 89. Bacillus of tetanus with spores (X 1000) (Frankel and 



Pfeiffer). 



killed off all the other bacteria, but the spores of tetanus 

 escaped and these then vegetated. 



Staining. All the ordinary stains, Gram's method also, 

 the spores being colored in the usual way. 



Pathogenesis. A small amount of the pure culture injected 

 under the skin of experiment animals will cause, in two to 

 three days, death from true tetenus, the tetanic condition 

 starting from the point of infection. At the autopsy nothing 

 characteristic or abnormal is found, and the bacilli have dis- 



