IQ4 ESSENTIALS OF BACTERIOLOGY 



Gelatin Plates. In the course of three days little round, 

 white colonies, which seldom increase in size, having a rough 

 border and very finely granulated. 



Stab-cultures. A very delicate 'gray line along the needle- 

 track, which does not become much larger. 



A gar Stroke Culture. A moist, grayish-colored skin, more 

 appreciable at brood-heat. 



Potato. At 37 C., after several days, a very thin, trans- 

 parent growth. 



Sugar Broth. Acid fermentation, no gas. 

 Indol is formed. 



Staining. Methylene-blue gives the best picture. Gram's 

 method is not applicable. As the bacillus is easily decol- 

 orized, anilin-oil is used for dehydrat- 

 ing tissue sections, instead of alcohol. 



Pathogenesis. Feeding the fowls with 

 the bacilli or injecting them under the 

 skin will cause death in from twelve to 

 twenty-four hours, the symptoms pre- 



Fig. 98. Chick- ceding death being those of a severe 

 en cholera in blood 77 . 



(X 1000) (Frankel septicemia. 



and Pfeiffer). The bacillus is then found in the blood 



and viscera and the intestinal discharges, 

 the intestines presenting a hemorrhagic inflammation. 



Guinea-pigs and sheep are immune. Mice and rabbits are 

 affected in the same manner as the fowls. 



Immunity. Pasteur, by injecting different-aged cultures 

 into fowls, produced in them only a local inflammation, and 

 they were then immune. But as the strength of these cul- 

 tures could not be estimated, many fowls died and the healthy 

 ones were endangered from the intestinal excretions, which is 

 the chief manner of infection naturally, the feces becoming 

 mixed with the food. 



Bacillus of Erysipelas of Swine (Loffler, Schiitz). 

 Synonyms. Schweinerotlauf bacillus (German); Rouget du 

 Pore (French). 



Origin. Found in the spleen of an erysipelatous swine by 

 Loffler in 1885. 



