2l6 ESSENTIALS OF BACTERIOLOGY 



Explanation of Noguchi Modified. Requires six tubes : 



In i and 2, one drop serum to be tested; in 3 and 4, one 

 drop known syphilitic serum; in 5 and 6, one drop normal 

 serum. 



To each tube add i c.c. i per cent, suspension washed 

 human blood-corpuscles, and o.i c.c. 40 per cent, fresh 

 guinea-pig serum (complement). 



Into 1,3, and 5, one drop antigen solution. 



Incubate at 37 C. one hour, then add 2 units antihuman 

 amboceptor to each tube. Incubate two hours and read 

 reaction every hour for next ten hours, keeping tubes at 

 room temperature. 



Tubes 2, 4, 6, complete hemolysis. 



Tube 5, complete hemolysis. 



Tube 3, no hemolysis. 



Tube i, no hemolysis. 



Results of Wassermann Test. Eighty per cent, of primary 

 cases give a positive result, but a negative reaction in this 

 stage does not mean much, as nearly 20 per cent, of cases are 

 negative. 



95 per cent, of secondary cases give a positive reaction. 



85 per cent, of tertian positive. 



90 per cent, congenital forms strongly positive. 



100 per cent, general paresis positive. 

 50 per cent, locomotor ataxia positive. 

 65 per cent, latent tertiary forms. 



Luetin Reaction (Noguchi). An emulsion of a pure 

 culture of the spirochetes of syphilis heated to 60 C. for 

 one hour, and made sterile, is called luetin. 



When applied subcutaneously by means of a fine needle, 

 an erythema lasting forty-eight hours results in normal 

 persons, but in persons affected with tertiary, latent, and 

 congenital syphilis after forty-eight hours a small induration 

 or papule appears, which at times becomes vesicular and 

 pustular, increasing in redness and turning bluish red in 

 three or four days. It is an adjunct to other tests for syphilis. 



