BUDS, GEMMULES. 



yeast plant formed ? Do they result from the growth 

 of the external envelope with which they are certainly 

 for a time connected, or is the soft diffluent material 

 within concerned in their production ? This question 

 can be answered conclusively from direct observation. 

 Never do we find a bud which does not contain some 

 of the soft diffluent germinal matter ; never one com- 

 posed of t/ie matter of the envelope only (Figs. 7, 8, 

 Plate I.). In the formation of these buds a very small 

 portion of the soft material protrudes through minute 

 pores in the envelope, perhaps pushing a very thin 

 layer of this latter before it, through which it imbibes 

 nutrient matter from around. It soon increases in 

 size forming a little nodule or button which remains 

 for a time connected with the parent mass by a very 

 narrow pedicle (Fig. 8, Plate I.). This pedicle con- 

 sists externally of matter like that of which the 

 envelope or cell-wall is composed, but in its centre 

 may be traced a very thin line of bioplasm, by which 

 the bioplasm or germinal matter of the bud remains 

 for a time connected with the parental bioplasm. 

 This line cannot be discerned in every instance, but 

 from the numerous observations I have made I do not 

 believe it is ever absent at this period of growth. I 

 have seen the diverticulum of germinal matter in so 

 many cases already projecting from the general mass, 

 that I feel sure the above description is correct. When 

 multiplication is going on with great rapidity, the mode 

 of formation of the buds may in fact be demonstrated 



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