SECTION III. 



PRINCIPLES OF STERILISATION AND PURE 

 CULTIVATION. 



CHAPTER X. 

 METHODS OF STERILISATION. 



71. Sterilising-. 



To sterilise an object, e.g. a nutrient solution, piece of apparatus, 

 &c., means to treat it in such a manner that it no longer contains 

 any living germs, and is therefore sterile. 



The reader must not expect to find in the present work a 

 detailed description of even the most important of the methods 

 of working adopted for this purpose. Those who have an oppor- 

 tunity of studying the methods of Technical Mycology in a 

 laboratory devoted to Fermentation Physiology will learn all 

 they need much more speedily and intelligibly from oral in- 

 struction than from a printed book. On the other hand, those 

 who have access to the latter only will attain their object by 

 the exertion of a little diligence in consulting the books referred 

 to later on, and especially 



Hueppe, Ferdinand : Die Metlioden der Balderien-Forsclmng, 

 5th edition, 1891, Wiesbaden (C. W. Kreidel). 



Lindner, Paul : Mikroskopisclie Betriebskontrolle in den 

 Garungsgewerben, 1895, Berlin (P. Parey). 



In the first-named compendium the reader will find a better 

 description than the present author could give of all the methods 

 used in general Microbiology. The second, very useful, work, 

 treats, with great experience, a narrower field, wherein it will 

 afford reliable guidance and help to the student on all matters 

 relating to fermentation technology. In the newest edition (1895) 

 of the work on water-analysis by TIEM ANN-GARTNER (I.), already 

 referred to (and which should be in every efficient chemical 

 laboratory), the reader will also find descriptions of the most 

 important manipulations and methods employed in sterilisation, 

 pure cultivation, re-inoculation, &c. In selecting apparatus for 

 installing a new laboratory for Fermentation Physiology work, 

 the beginner should seek the advice of an expert, and should 



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