CONTENTS 



CHAPTER PAGES 



I. CULTURAL TECHNIQUE 1-36 



Glassware 1 



Cleaning glassware 2 



Sterilization of glassware 3 



Preparation of media 4 



Ingredients of media 5 



Reaction of media 5 



Neutralization of media . . 6 



Filtering of media 7 



Plugging tubes 9 



Sterilization of media 



Care of media . . < . . 11 



Preparation of broth 12 



Preparation of gelatin 14 



Preparation of agar 15 



Preparation of milk * 16 



Preparation of sugar media . 17 



Preparation of litmus solution 18 



Preparation of water blanks 18 



Quantitative analysis of milk ..19 



Qualitative analysis of milk 29 



Isolation of pure cultures 30 



Platinum needles t 30 



Plate cultures - 31 



Study of plate cultures 32 



Test-tube cultures 33 



Study of test-tube cultures 35 



II. MICROSCOPICAL TECHNIQUE 37-52 



The microscope and its accessories 37 



Slides and cover glasses 41 



Staining solutions 42 



