APPENDIX B 



18. Best media for long-continued growth . 



19. Quick tests for differential purposes 



131 



HI. PHYSICAL AND BIOCHEMICAL FEATURES 



1. Fermentation tubes con- 

 taining peptone water or 

 sugar-free bouillon and 



Gas production, in per cent 



Growth in closed arm 



Amount of acid produced in 1 d. 



Amount of acid produced in 2 d. 



Amount of acid produced in 4 d. 



2. Ammonia production, feeble, moderate, strong, absent, masked by 



acids. 



3. Nitrates in nitrate broth, reduced, not reduced. 



Presence of nitrites , ammonia 



" " nitrates , free nitrogen 



4. Indol production, feeble, moderate, strong. 



5. Toleration of acids, great, medium, slight. 



Acids tested 



6. Toleration of NaOH, great, medium, slight. 



7. Optimum reaction for growth in bouillon, stated in terms of 



Fuller's scale 



8. Vitality on culture media, brief, moderate, long. 



9. Temperature relations : 



Thermal death point (10 minutes' exposure in nutrient broth 

 when this is adapted to growth of organism) C. 



Optimum temperature for growth C., or best growth 



at 15 C., 20 C., 25 C., 30 C., 37 C., 40 C., 50 C., 60 C. 



