146 



EXPERIMENTAL DAIRY BACTERIOLOGY 



Gram's stain, 43 

 Gram's solution, 47 



Hanging block, 51 

 Hanging drop, 49 

 Heat, effect on milk, 89 

 Heated milk, detection of, 91 

 Homogeneous milk, 90 



Incubation, temperature of, 23; 



time of, 23 

 Indol, test for, 72 

 Inhibition, 20 

 Iodine solution, 43 

 lodoform, 79 



Leucocytes, 116, 117 



Light, 39 



Litmus, 5 ; preparation of, 18 



Litmus lactose agar, 29 



Litmus lactose gelatin, 29 



Litmus milk, 17 



Lugol's solution, 43 



Manure, contamination of milk 

 from, 57 



Meat infusion, 5 



Media, care of, 11 ; clearing of, 16; 

 filtering of, 7 ; ingredients of, 5 ; 

 neutralization of, 6 ; reaction of, 

 5; sterility of, 11, 17; steriliza- 

 tion of, 9 ; sugar-free, 18 



Methylene blue, 43 



Micrometer, ocular, 51 ; stage, 52 



Micron, 52 



Microscope, 37 ; accessories of, 37 



Milk, acid fermentation of, 76 ; 

 acidity of , 16 ; agar, 75 ; alcoholic 

 fermentation of, 78 ; analysis of, 

 19, 29; bitter fermentation of, 

 80 ; butyric fermentation of, 81 ; 

 condensed, 88 ; contamination of, 

 53 ; cycle of fermentations in, 80 ; 

 determination of acidity of, 76 ; 

 digestion of, 77 ; dilution of, 21 ; 

 examination of : for diphtheria, 

 112; for fibrin, 122; for leu- 

 cocytes, 117, 120; for pyogenic 

 bacteria, 114, 124; for tubercu- 

 losis, 112, 114 ; for typhoid, 112 ; 

 homogeneous, 90; hygiene of, 

 110; microscopical examination 



of, 116; pasteurization of, 89; 

 preservation of, 86 ; ropy, 79 ; 

 slimy, 79; sweet-curdling of, 77 



Needles, 30 ; Ravenel's, 31 ; sterili- 

 zation of, 31 

 Nessler solution, 71 

 Nitrites, test for, 71 



Object slides, 41 ; cleaning of, 41 

 Oidium lactis, 81 

 Oil-immersion objective, 40 

 Oxygen, relation of bacteria to, 72 



Pasteurization, 89 



Pedesis, 50 



Pepsin, 104 



Peptone, 5 ; solution of, 72 



Physiological salt solution, 18 



Pipettes, calibration of, 125 ; prep- 

 aration of, 125 ; sterilization of, 4 



Plate cultures, 31 ; counting of, 22 ; 

 incubation of, 23 ; study of, 32 



Preservatives, 87 



Pure cultures, isolation of, 30 



Pus cells, 116 



Qualitative analysis, 29 



Quantitative analysis, 19, 26 ; accu- 

 racy of, 26 ; comparative method, 

 28 ; expression of results, 25 



Rennet, 104 

 Ropy milk, 48 



Sanitary milk, plan for preparation 

 of, 63 



Separator slime, examination of, 

 94 



Shake cultures, 69 



Spore stain, 48 



Spores, test for, 73 



Stab cultures, 34 ; study of, 35 



Stained preparations, examination 

 of, 46 ; making of, 44 ; mounting 

 of, 45 



Staining solutions, 42 



Staphlococcus pyogenes aureus, 116 



Starters, 97 ; flavor-producing prop- 

 erties of, 100 ; preservation of 

 properties of, 100 ; purity of, 97 ; 

 vitality of, 99 



