THE GENUS SARCINA 229 



growth, and three only a meager one. In dextrose broth 

 twenty-four strains gave an alkalin reaction, eighty-five 

 produced an acidity below .004 normal, twenty an acidity 

 between .005 and .009, and eight a higher acidity. In lac- 

 tose broth forty-one strains formed no acid, eighty-four 

 an acidity under .004 normal, and nine an acidity be- 

 tween .005 and .009, while three were over .010. Forty- 

 three cultures grew better at 20 degrees than at 37 degrees, 

 and only twelve, better at 37 degrees than at 20 degrees. 

 Forty-nine showed better color production at 20 degrees, 

 and in eighty-two cases chromogenesis was equal at both 

 temperatures. In forty-four cases the color formed could 

 be matched under the Light Cadmium Yellow column 

 of the Frontispiece, in sixty-six cases under Medium 

 Cadmium Yellow, and in twenty-one cases under Orange 

 Yellow. Forty-seven strains failed to liquefy gelatin. 

 Of the remainder, thirteen produced a liquefaction less 

 than .5 centimeter deep in two weeks, twenty-five a lique- 

 faction .6 to 1.0 centimeter deep, twenty-three a liquefac- 

 tion 1.1 to 1.5 centimeters deep, twenty a liquefaction 

 1.6 to 2.0 centimeters deep, and eight a liquefaction over 

 2.1 centimeters. 



In addition to these characters, it may be noted that 

 Gordon (1906) records the failure of all sarcinae examined 

 by him to ferment either lactose, maltose, glycerin, or 

 mannite. It is probable, therefore, that the fermentative 

 powers of the group are low in other carbohydrate media, 

 as in dextrose and lactose. 



