THK ASCOSPOHES. 



•33 



l)U(ls ;it :ill. J II the csise of Wortmiinii's wine yea«t Joltaniiui- 

 beig II., for exiimjde, this result Ciiu he uhtiiinetl hy phicing the 

 young cells in a sjitunit^d jKjueous solution of Ciilciuni suliilmte, 

 whoiehy l)U<Kling is suiipressetl iind s|>orulati(in hegins. C'oiu- 

 jiiue whiit has heen stated in the la-^t jNinigi-u^th hut one of 



rp 



riie progress of develoimient in the ascosiKM-es will now lie 

 followed in the ease of Sarr/i. rt-rcinititr I. Haun'-n. If, at the 

 end of ahout twenty-four iiours after the streak has been laid on 

 the blotrk and the latter placed in the thermostat at 25° C, a small 

 portion of the y»*:ist strt-ak he sfrat<'hed ofY with a clean glass roil 

 or a net'dlf. di>tributed in a siugle drop of water, and examined 



v.. 



fe ^<: 





^£ 'S^^'^^^i 



Km. 141.— \\iiie Veast (n>ni WalixinJu-im. 



Assemblage "' buds from an oM nim ; some u( the iiiemlMTs have produced spurea. 

 Magll. Soo (-1/Trr Adrrhnlil.) 



with a strong power (300 to 500) under a cover glass, decided in- 

 dications »)f incipient sporulation will l)e iihservalijc in a larger or 

 smaller numlier of the cell>. The plasma will he found to have 

 sepanited itself into a number of Uills, as shown at a-</ of 

 Fig. 142. Each of these globules soon becomes covere<l with a 

 membrane, so that ripe spores (/'-/') ^^'i^l '*»^ visible after another 

 twenty-four houis. \Vitli regard to the lu'haviour of the cell 

 nucleus during this periotl, fuller jKUticulars will be given in 

 ji 250. It may be remarked that a ]K)rtion of the plasma in the 

 parent-cell (ascus) remains over, and holds the spores somewhat 

 together. 



A careful .sorting of the samjiles will ijuickly show that con- 

 sidenible dilTerences exist with respect to the dimensions of the 

 spores, and that too not merely between those in ditTeient cells 



