THE OHANT'LHS. 



«57 



o*»\ 



/ 



,^^v^o^rr^ 



f 



i«j. 



^6„ 





in ;i loutinuuu.s [Jiotophismic liluuifiit, as ilepict4*<i in Vi^. 155. 

 Hieionymus calls this union 11 ceutnil lihiment. Subsfijut-ntly, 

 on the basis of tlio observations 

 of A. Gffirtz, the existence of 

 such an arrangenuMit of the 

 gi-anules was denied by A. ZlM- 

 MKUMANX (IV.), until L'usiij,'nin(li 

 coniirnied the accuracy of Hiero- 

 nynuis' discovi-ry, with the un- 

 important limitation that the 

 allo^rt'd refjularity of airanj^ement 

 is, in many cases, present Ui only 

 an inferior extent. 



Clearly under the influence of 

 Biitschli's theory uf the sti-ucture 

 of pi'otoplasm (p. 42, vol. i.), Jaxs- 

 8EXS and Lkbla.N'C (I.) explained 

 the appeaiances in (juestion as 

 the knots of the network observed 

 by them in the cytoplasm of the 

 yeast cell (Fii,'. 15O). In the 

 author's opinion, Imwever, it has 

 not yet been stiictly proved that 

 these knots are identical with the 

 structures here referred to as 

 granules. The existence of a 

 reticulated fi-imework in the 

 cytoplasm of the yeast cell hivs, 



neverthele.><s (accordiufx to a written communication), been also 



demonstrjited Ity II. Will. 



Staining the granules for the purpo.«<e of identification can 



be effected by tin- method recommended 



by P. EuN.sT (II.) and tested by Raum. 



The prepanition is treated with mcxler- 



ately warm (not boiling hot) Lu'tller- 



Methylene Hlue .solutinii, followed b\ 



Wiushint' with water, and liuallv by stain 



int; with a cold .solution of Bismarck 



lirown. This causes the granules to 



ai)pear black, ami to stand well out from 



the surrouniling brown-stained plasma. 



A ditYerential staining of the integument 



of the granules can be elTected by the 



method recommended by (.'a.sjignmdi. 



Here the yeast cells are fixed (Ji 34) with 



an alcoholic solution of conx>sivo sub- 

 limate, tlu' fatty contents of the granules are then exti-acte<l with 



absolute alcohol, the rest stained with a 20 per cent, solution of 



Fig. 155.— iiramiles 



ill a living cell of prfsst;tt yeut in ooune 

 ol Ixiildini;. .Sevi-rul •>( tlu- jnTiiiule» are 

 deciileilly inultiiiUKolHr. l»o <<t ihi-ni 

 iifiiiK also iiiiiih larger than tlu- rest. 



Tlu- twc. white areas repre-irnt vaeUi-le*. 



Mogu. 440.'. (•V'''' //iVri'Hi/i/iii*.) 



Kio. ij'.. iki^Mii r-<ii \ia»t 

 after a ftojoiirn of forty-four 

 hoiin III l>eer wort ; oiiscrvi-J 

 in the live state. Kxhlhit* the 

 |ila$nial framework, with ap- 

 purtenant kii'ts. nferreil t<.> 

 In the •• " 1 >'■ ■ ■ '1 '. 1. li-us 

 is not 

 (Ajtf, 



