frt CULTURES IX LIQUID MEDIA. 



tube for the purpose of starting a new culture. Or we may start a 

 pure culture from a drop of blood taken from the veins of an animal 

 which has been inoculated with anthrax, or any similar infectious 

 disease in which the blood is invaded by a bacterial parasite. 



But if we have not a pure culture to start with our liquid media 

 do not afford us the means of obtaining one ; and if two or more 

 bacteria which resemble each other in their morphology are associated 

 in such a culture we cannot differentiate them, and are likely to infer 

 that we have a pure culture of a single microorganism when this is 

 not really the case. 



But if we have pure stock to start with we may maintain pure 

 cultures in liquid media without any special difficulty. 



Various characters of growth, etc., are to be observed in culti- 

 vating different microorganisms in liquid media. Thus some grow 

 at the surface in the form of a thin film or membranous layer " my- 

 coderma " while others are distributed uniformly through the liquid, 

 rendering it opalescent or more or less milky and opaque ; others, 

 again, form little flocculi which are suspended in the transparent 



Fro. 35. 



fluid. Usually, when active growth has ceased, the bacteria fall to 

 the bottom of the tube as a more or less abundant, white or colored, 

 pulverulent or glutinous deposit. In some cases the liquid is colored 

 with a soluble pigment formed during the growth of the bacteria, 

 and usually this is formed most abundantly at the surface, where 

 there is free access of oxygen. The reaction of the medium is often 

 changed as a result of the growth of bacteria in it. From being neu- 

 tral it may become decidedly alkaline or acid in its reaction. These 

 changes may be observed by adding a litmus solution before sterili- 

 zation of the culture medium, and observing the change of color 

 when an acid-producing bacterium is under cultivation. The re- 

 ducing power of bacteria upon various aniline colors may also be 

 MU died ; also their power to break up various organic substances 

 shown by the evolution of gas or other volatile products which 

 may be collected, or by substances which remain in solution and 

 can !><> studied by ordinary chemical methods. 



Drop Cultures. When we desire to study the life history of a 

 microorganism and to witness its development from spores, for ex- 

 ample, its motions, etc., the method of cultivation in a hanging drop 



