186 ACTION OP SALTS. 



i, but was fatal to Micrococcus tetragenus two hours' exposure. 

 K . >ch found that a five-per-cent solution failed to destroy anthrax 

 spores in six days. Exposure to a twenty-per-cent solution for forty- 

 eight hours does not destroy the virus of symptomatic anthrax (Ar- 

 loing, Cornevin, and Thomas). In the experiments of Jager immer- 

 sion in a solution of 1 : 3 destroyed the infective virulence of certain 

 pathogenic bacteria (fowl cholera, rothlauf, glanders), as tested by 

 injection into mice, but failed to kill anthrax spores and tubercle ba- 

 rilli. The antiseptic power of ferrous sulphate is placed by Miquel 

 at 1 : 90. In the writer's experiments 1 : 200 prevented the develop- 

 ment of micrococci and of putrefactive bacteria in bouillon placed 

 in the incubating oven for forty-eight hours. Leitz found that a 

 five-per-cent solution required three days' exposure for the destruc- 

 tion of the typhoid bacillus. 



Gold Chloride. Antiseptic in the proportion of 1 : 4,000 (Miquel). 

 Boer has made extended experiments with the chloride of gold and 

 sodium. We give his results below. In his disinfection experi- 

 ments a bouillon culture which had been in the incubating oven for 

 t \\ nty-four hours was used, and the time of exposure was two hours. 



Lead Chloride. Antiseptic in the proportion of 1 :500 (Miquel). 



Lead Nitrate. Antiseptic in the proportion of 1 : 277 (Miquel). 



Lithium ( 1 ll<tru1<>. Antiseptic in the proportion of 1 : 11 (Mi- 

 quel). 



Mangam *< rrotochloride. Antiseptic in the proportion of 1:40 

 (Miquel). 



Mercuric Chloride. Koch's experiments (1881) gave the follow- 

 ing results : A solution of 1 : 1,000 destroys anthrax spores in a few 

 minutes, and 1 : 10,000 is effective after a more prolonged exposure. 

 The writer (1884) obtained similar results 1 : 10,000 destroyed the 

 Bpo res of Bacillus anthracis and of Bacillus subtilis in two hours. 

 More recent experiments indicate that failure to grow in culture so- 

 1 nt ions cannot be accepted as evidence of the destruction of vitality 

 in the case of spores exposed to the action of this agent, unless due 

 pi "cautions ;m> t;ik-n to exclude the restraining influence of the small 

 amount of mercuric chloride which remains attached to the spores. 

 l, : ,,i ;l -< .-itainrd that the development of spores is restrained by 



