PYOGENIC BACTERIA. 



281 



a pure culture macje directly into the circulation, and there is evi- 

 dence that the pathogenic potency of this micrococcus may vary 

 considerably as a result of conditions relating to its origin and culti- 

 vation in the animal body or in artificial media. When injected in 

 considerable quantities it may be obtained in cultures from the 

 urine, but not sooner than six or eight hours after the injection, and 

 not until the formation of purulent foci in the kidneys has already 

 occurred (Wyssokowitsch). 



The pyogenic properties of this micrococcus have been demon- ^ 

 strated upon man by the experiments of G-arre, of Bockhart, and of 

 Bumm. The first-named observer inoculated a small wound at the 

 edge of one of his finger nails with a minute quantity of a pure cul- 

 ture, and a subepidermal, purulent inflammation extending around 



FIG. 81. Vertical section through a subcutaneous abscess caused by inoculation with staphylo- 

 cocci, in the rabbit, forty-eight hours after infection; margin towards the normal tissue, x 950. 

 (Baumgarten.) 



the margin of the nail resulted from the inoculation. Staphylococ- 

 cus aureus was recovered in cultures from the pus thus formed. A 

 more extensive and extremely satisfactory experiment was subse- 

 quently made by Garre, who applied a considerable quantity of a 

 pure culture obtained from the above-mentioned source third gene' 

 ration to the uninjured skin of his left forearm. At the end of 

 four days a large carbuncle, surrounded by isolated furuncles, de- 

 veloped at the point where the culture had been applied. This ran 

 the usual course, and it was several weeks before it had completely 

 healed. No less than seventeen scars remained to give evidence of 

 the success of the experiment. 



In Bockhart's experiments a similar but milder result was ob- 

 tained, the conditions having been somewhat different. A small 



