NOT DESCRIBED IN SECTIONS IV. AND V. 331 



of agar, at 37 C., a grayish- white or yellowish layer is formed at the end of 

 twenty-four hours, which has a varnish-like lustre. Upon potato, at 30 to 

 35 C. , at the end of forty-eight hours a white or yellow layer has de- 

 veloped. 



Pathogenesis. According to Heydenreich, inoculations in rabbits, dogs, 

 chickens, horses, and sheep cause a skin affection which is identical with 

 that which characterizes Biskra button in man. When rubbed into the 

 healthy skin of man it also produces the development of abscesses. 



27. MICROCOCCUS OP DEMME. 



Synonym. Diplococcus of pemphigus acutus (Demme). 



Obtained by Demme (1886) from the contents of the bullae in a case of 

 pemphigus. 



Morphology. Micrococci of from 0.8 to 1.4 // in diameter; usually united 

 in pairs resembling the " gonococcus " and having a length of 1.8to3/*, 

 very opaque and not surrounded by a capsule ; usually associated in irregu- 

 lar masses. 



Biological Characters. Aerobic micrococci. Do not grow at the room 

 temperature. Upon agar plates, at 37 C., at the end of thirty-six to forty- 

 eight hours milk-white, spherical colonies, which project above the surface, 

 are developed ; later club-shaped outgrowths form around the periphery of 

 the colony, giving it the appearance of a rosette, or sometimes of a bunch of 

 grapes. At the end of two weeks the surface is covered with smooih projec- 

 tions and has a cream-like color. In streak cultures upon agar a similar 

 growth occurs along the impfstrich, having club-like projections and stalac- 

 tite-like outgrowths. Growth also occurs upon potato at a temperature of 

 37 C. This micrococcus develops slowly in the incubating oven, and 

 scarcely any growth occurs at a temperature below 32 C. 



Pathogenesis. The injection of a pure culture into the lungs of guinea- 

 pigs gave rise to emaciation and debility and to the formation of foci of 

 broncho-pneumonia, the size of a pea, in the lungs. 



28. STREPTOCOCCUS OF MANNEBERG. 



Obtained by Manneberg (1888) from the urine in acute cases of Bright's 

 disease. 



Morphology. Micrococci, aboutO.9// in diameter, solitary, in pairs, or 

 in chains of six to ten elements. Does not differ in morphology from Strep- 

 tococcus pyogenes. 



Stains with the usual aniline colors and also by Gram's method. 



Biological Characters. An aerobic and facultative anaerobic micro- 

 coccus, which slowly produces a viscid softening of nutrient gelatin. Grows 

 in the usual culture media at the room temperature. In gelatin stick cul- 

 tures forms a white stripe along the line of puncture, which consists of small 

 colonies. At the end of three or four weeks a funnel is formed containing 

 very viscid liquefied gelatin, and at the same time brush-like outgrowths are 

 seen along the line of development. Upon the surf ace of agar the growth 

 resembles that of Streptococcus pyogenes, but is somewhat more abundant. 

 IJpon potato, at 37 C., at the end of four or five days white, drop-like colo- 

 nies are developed of about 0.5 millimetre in diameter; these become con- 

 fluent and form a slimy layer. Milk becomes strongly acid and coagulates 

 within twelve hours when inoculated with this micrococcus. 



Pathoge nesis. Subcutaneous injection of 0.75 to 1 cubic centimetre 

 causes the formation of a local abscess in dogs and rabbits. Intravenous 

 injections produce inflammatory changes in the kidneys ; at the end of three 

 or four days the urine contains red blood corpuscles, renal epithelium, blood 

 casts, albumin, and streptococci. 



