420 



BACILLI IN CHRONIC INFECTIOUS DISEASES. 



can easily be obtained in pure cultures from the interior of suppurat- 

 ing nodules and glands which have not yet opened to the surface, 

 and the same material will give successful results when inoculated 

 into susceptible animals. But the discharge from the nostrils or from 

 an open ulcer contains comparatively few bacilli ; and as these are 

 associated with various other bacteria which grow more readily in 

 our culture media, it is not easy to obtain pure cultures, by the plate 

 method, from such material. 



In the guinea-pig subcutaneous inoculation is followed in four or 

 five days by tumefaction at the point of inoculation, and after a time 

 ;i prominent tumor with caseous contents is developed ; ulceration of 

 the skin follows, and a chronic, purulent ulcer with irregular, indu- 

 rated margins results ; after a time this may cicatrize. Meanwhile 

 the lymphatic glands become involved, and the symptoms of general 



Fio. 126. Section through a glanders nodule in liver of field mouse. Tissue x 250. Bacilli 

 X 600. (Baumsarten.) 



infection are developed at the end of four or five weeks ; the glands 

 suppurate, and in males the testicles are also involved ; finally a dif- 

 fuse inflammation of the joints occurs, and death results from ex- 

 haustion. In the guinea-pig the specific ulcers upon the nasal mu- 



