IN SUSCEPTIBLE ANIMALS. 



449 



a central light-brown nucleus ; at the end of ten days the deep colonies are 

 still quite small, of a brown color, and opaque. 



In glycerin-agar roll tubes, at the end of twenty-four hours, the deep colo- 

 nies are in the form of a Biconvex lens, and appear spherical when viewed 

 in face and biconvex when seen from the side ; they have a straw color 

 by transmitted light and are bluish- white by reflected light ; the superficial 

 colonies are translucent, with a bluish white lustre, 



On potato, at 22 C., at the end of forty eight hours there is a thin, dirty- 

 yellow growth of limited extent; at the end of ten days there is a thin, 

 gamboge yellow layer and little masses of the same color; the growth is 

 quite thin, with irregular outlines, and is confined to the vicinity of the 

 impfstrich. \ 



Grows in nutrient agar containing 0.2 per cent of hydrochloric acid. 

 Thermal death point 55 C. Grows in agua coco without forming gas, and 

 causes this liquid and bouillon to become slightly translucent not milky. 



Pathogenesis. Pathogenic for guinea-pigs, less so for rabbits. Guinea- 

 pigs inoculated subcutaneously with a few drops of a pure culture die in ten 

 or twelve hours from general infection. There is usually a considerable 

 effusion of bloody serum in the vicinity of the point of inoculation, and the 

 spleen is more or less enlarged. 



71. BACILLUS CRASSUS SPUTIGENUS, 



Obtained by Kreibohm (1886) from the sputum of two individuals, and 

 once in scrapings from the tongue. 



Morphology. Short, thick bacilli, of oblong form, with rounded corners, 

 en bent or twisted "sausage-shaped." Immediately after division the 

 bacilli are about one-half longer than they are broad, but before dividing 



FIG. 141. Bacillus crassus sputigenus, from blood of mouse, x 700. (Flug^e.) 



again they may attain a length of three to four times the breadth. Irregular 

 forms with swollen ends or uneven contour are frequently seen. 



This bacillus is quickly stained by the ordinary aniline colors and also 

 by Gram's method. 



Biological Characters. An aerobic, non-liquefying (non-motile ?) ba- 

 cillus. Grows in various culture media at the room temperature more 

 rapidly in the incubating oven. "Appears to form spores at 65 U. 

 (Fliigge). 



In gelatin plates, at the end of thirty-six hours, grayish- white colonies are 

 developed, which soon reach the surface of the gelatin and spread out as 



