484 PATHOGENIC AEROBIC BACILLI 



very markedly in this medium from any other bacilli examined. The same 

 changes are to be seen at room temperature, but more slowly. 



Egg- Albumin: Plain. Twenty four hours at 37 C., yellowish-white, 

 very prof use growth all along the needle track ; yellowish-green spreading 

 out liom it almost to sides of tube, and in the condensation water as well. 

 The growth has no especial distinguishing characteristics. Irregular lique- 

 faction occurs, but the growth at no time differs in any marked way from 

 other varieties of the Bacillus pyocyaneus. 



Blood Serum. Twenty-four hours at 37 C. shows flat, moist colony 

 with bluish-green fluorescence in its neighborhood. Liquefaction begins 

 early and goes on slowly until complete in from one to two weeks, with an 

 increasing intensity of color which becomes markedly blue, and eventually 

 almost black. 



Milk. Behaves as do the other bacteria. 



BEHAVIOR TO TEMPERATURE. Grows at 15-25 C. slowly; much more 

 freely at 35 J -38 C., when it produces the coloi more quickly. 



RAPIDITY OF GROWTH. Moderate. 



SPORE-PRODUCTION. Not observed. 



NEED OF AIR. Does not grow under mica. Facultatively anaerobic, but 

 does not produce color except with free access of oxygen. 



GAS-PRODUCTION. Produces faint foul odor. 



BEHAVIOR TO GELATIN. Liquefies gelatin slowly. 



COLOR-PRODUCTION. Produces a bluish-green color which in old cul- 

 tures changes almost to a black. Upon the addition of acids (both vegetable 

 and mineral) to cultures the color changes to red, and upon the addition of 

 alkalies a bright grass-green appears. This reaction is best seen in bouillon 

 and gelatin cultures, but occurs in other media as well, notably blood-serum. 



BEHAVIOR TO ANILINE DYES. Stains easily and well with any of the 

 aniline dyes usually employed, and by Gram's method. 



MICROSCOPIC APPEARANCE IN DIFFERENT MEDIA. Under the micro- 

 scope, its general appearance on various media is of a rod larger than the 

 Bacillus pyocyaneus. In peptone cultures this difference is verv marked. 

 In this case, the Bacillus pyocyaneus tested appeared as very short, oval, 

 bacilli, almost like micrococci, while the new bacillus showed as a long, 

 fine rod, from four to six times as long as broad length about one-half the 

 diameter of a red-blood corpuscle and arranged sometimes two or three 

 end to end. These same cultures transferred to gelatin became indistin- 

 guishable from each other in size. 



PATHOGENESIS. Injections of small quantities (0.5 centimetre) of a bouil- 

 lon culture twenty-four hours old into the abdominal cavity of rabbits and 

 guinea-pigs, killed fifty per cent in from twenty-four to thirty-six hours. 

 Autopsy showed general congestion of abdominal viscera, slight effusion into 

 the peritoneal cavity, and cover-glass preparations and cultures showed the 

 bacilli in the effusion in the abdominal cavity, as well as in the blood from 

 the heart and various organs. 



96. BACILLUS OP FIOCCA. 



Found by Fiocca in the saliva of cats and dogs. 



Closely resembles the influenza bacillus of Preiffer and of Canon. 



Morphology. Resembles the bacillus of rabbit septicaemia, but is only 

 half as large from 0.2 to 0.33 n in breadth. The length is but little greater 

 than the breadth. Usually seen in pairs, closely resembling diplococci. 

 When cultivated on potato it appears to be a micrococcus, but in the blood 

 of infected animals and in bouillon cultures it is seen to be a short bacillus. 



Stains with difficulty with the usual aniline colors, but is readily stained 

 by Ehrlich's method or with Ziehl's solution. 



Biological Characters. An aerobic and facultative anaerobic, non- 

 liquefying, non-motile bacillus. Spore formation not observed. Grows best 



