496 PATHOGENIC AEROBIC BACILLI 



somewhat irregular outlines; later liquefaction commences and crater-like 

 depressions in the gelatin are formed, in which a gas bubble is seen ; com- 

 plete liquefaction occurs in the course of a few days. In gelatin stick cul- 

 tures liquefaction occurs all along the line of inoculation, and is complete 

 at the end of from eight to fourteen days. Upon agar plates the colonies 

 are granular in the centre, and the margins, under a low power, are seen to 

 be fringed. Upon the surface of agar a moist, slimy layer is formed along 

 the impfstrich. Upon, potato, at the end of twenty-four hours, a yellowish- 

 brown layer is formed. Upon blood serum development is rapid in the form 

 of a whitish layer, which extends over the whole surface. The cultures, 

 and especially those kept in the incubating oven, give off a disagreeable 

 putrefactive odor, which is most intense in the blood-serum cultures. 



Pathogenesis. Pathogenic for mice. When injected subcutaneously 

 into rabbits it gives rise to intense local inflammation and progressive gan- 

 grene of the connective tissue. 



112. BACILLUS OF LUMNITZER. 



Obtained by Lumnitzer (1888) from the bronchial secretions of persons 

 suffering from *' putrid bronchitis." Etiological relation not demonstrated. 



Morphology. Bacilli with round ends, from 1.5 to 2n long, somewhat 

 curved. 



Stains with the usual aniline colors. 



Biological Characters. An aerobic, motile bacillus. Does not grow in 

 nutrient gelatin at the room temperature. Grows slowly upon agar and 

 more rapidly upon blood serum at 36 to 38 C. Forms spores. Upon agar 

 plates, at 37 C , small, grayish-white colonies are formed in two or three 

 uays; upon the surface these form hemispherical masses which slowly in- 

 crease in size. At the end of six or seven days the cultures give off a dis- 

 agreeable odor, quite like that given off by the sputum of the cases of putrid 

 bronchitis from which the bacillus was obtained. Upon the surface of 

 blood serum the growth is rapid and forms grayish-white, shining colonies, 

 of about one millimetre in diameter, which become confluent at the end of 

 about four days, and cover the entire surface in eight or nine days. 



Pathogenesis. Causes a purulent inflammation when injected into the 

 lungs of rabbits, which involves the bronchial tubes, the blood vessels, and 

 the pulmonary alveoli ; when injected subcutaneously produces inflamma- 

 tion and necrosis of the tissues. 



113. BACILLUS OF TOMMASOLI. 



Obtained by Tommasoli (1889) from the hairs of the head of a patient suf- 

 fering from a form of sycosis supposed to be due to the presence of this 

 parasite (?). 



Morphology. Short, straight bacilli, with round ends, from 1 to 1.8 /* 

 long- and from 0.25 to 0.3 n broad ; often united in chains containing four 

 to six elements. 



Stains with the usual aniline colors. 



Biological Characters. An aerobic, non-liquefying, non-motile bacil- 

 lus. Does not form spores. Grows slowly at the room temperature in the 

 usual culture media. Upon gelatin plates, at the end of four days, the deep 

 colonies are seen as small, white points, the superficial colonies as smooth 

 discs of a grayish color. At the end of a month the deep colonies may be as 

 large as a mustard seed; the superficial are thin, shining, and slimy, and 

 have a diameter of one to two millimetres. In gelatin stick cultures a con- 

 vex, shining, white mass is developed at the point of inoculation, and along 

 the line of puncture in the course of five or six days a white line of growth 

 is seen which consists of closely crowded, small colonies. Upon agar the 

 development is very slow, and forms at first thin, slimy, grayish-white 

 patches which are distributed along the impfstrich ; later these become con- 



